ac4C acetylation regulates mRNA stability and translation efficiency in osteosarcoma
نویسندگان
چکیده
ObjectiveN4-acetylcytidine (ac4C) acetylation can promote target gene expression through improved mRNA stability. To explore the role of ac4C in osteosarcoma, U2OS and MG63 cell lines were treated with N-acetyltransferase 10 (NAT10) inhibitor Remodelin. Reverse transcription–polymerase chain reaction (RT-PCR) Western blot used to test protein efficiency.MethodsThe proliferation rate osteosarcoma cells was measured by a counting kit-8 (CCK8) assay. The cycle apoptosis analyzed flow cytometry. invasiveness detected transwell invasion genes screened acetylated RNA immunoprecipitation sequencing (acRIP-seq).ResultsWe found that when Remodelin at optimal concentration, their NAT10 inhibited, G1 phase increased (P < 0.05) but those S decreased, apoptotic early late stages increased, decreased 0.05).ConclusionsThe farnesyltransferase subunit beta (FNTB) identified acRIP-seq as one further verified RT-PCR analyses. demonstrated reduce stability translation efficiency cells. In conclusion, inhibition block metastasis well arrest. Ac4C contributes downstream mRNA.
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ژورنال
عنوان ژورنال: Heliyon
سال: 2023
ISSN: ['2405-8440']
DOI: https://doi.org/10.1016/j.heliyon.2023.e17103